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Int J Mol Sci. 2017 Jan 17;18(1). pii: E175. doi: 10.3390/ijms18010175.

Development and Sequential Analysis of a New Multi-Agent, Anti-Acne Formulation Based on Plant-Derived Antimicrobial and Anti-Inflammatory Compounds.

Author information

1
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. crina.saviuc@yahoo.com.
2
S.C. SANIMED INTERNATIONAL IMPEX S.R.L., Sos. Bucuresti-Magurele, nr.70, Sector 5, 051434 Bucharest, Romania. crina.saviuc@yahoo.com.
3
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. ciubuca.b@gmail.com.
4
S.C. SANIMED INTERNATIONAL IMPEX S.R.L., Sos. Bucuresti-Magurele, nr.70, Sector 5, 051434 Bucharest, Romania. ciubuca.b@gmail.com.
5
S.C. Biotehnos S.A., Gorunului Str. 3-5, 075100 Otopeni, Ilfov, Romania. gabi.dinca@biotehnos.com.
6
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. cbleotu@yahoo.com.
7
Ştefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, Sector 3, 030304 Bucharest, Romania. cbleotu@yahoo.com.
8
S.C. Biotehnos S.A., Gorunului Str. 3-5, 075100 Otopeni, Ilfov, Romania. drume_67@yahoo.com.
9
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. carmen_balotescu@yahoo.com.
10
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. bmarcelica@yahoo.com.
11
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. gratiela87@gmail.com.
12
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. lumidascalu@yahoo.com.
13
Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania. veronica.lazar2009@gmail.com.

Abstract

The antibacterial and anti-inflammatory potential of natural, plant-derived compounds has been reported in many studies. Emerging evidence indicates that plant-derived essential oils and/or their major compounds may represent a plausible alternative treatment for acne, a prevalent skin disorder in both adolescent and adult populations. Therefore, the purpose of this study was to develop and subsequently analyze the antimicrobial activity of a new multi-agent, synergic formulation based on plant-derived antimicrobial compounds (i.e., eugenol, β-pinene, eucalyptol, and limonene) and anti-inflammatory agents for potential use in the topical treatment of acne and other skin infections. The optimal antimicrobial combinations selected in this study were eugenol/β-pinene/salicylic acid and eugenol/β-pinene/2-phenoxyethanol/potassium sorbate. The possible mechanisms of action revealed by flow cytometry were cellular permeabilization and inhibition of efflux pumps activity induced by concentrations corresponding to sub-minimal inhibitory (sub-MIC) values. The most active antimicrobial combination represented by salycilic acid/eugenol/β-pinene/2-phenoxyethanol/potassium sorbate was included in a cream base, which demonstrated thermodynamic stability and optimum microbiological characteristics.

KEYWORDS:

acne; antimicrobial; eucalyptol; eugenol; limonene; plant-derived compounds; β-pinene

PMID:
28106736
PMCID:
PMC5297807
DOI:
10.3390/ijms18010175
[Indexed for MEDLINE]
Free PMC Article

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