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J Clin Lab Anal. 2017 Nov;31(6). doi: 10.1002/jcla.22130. Epub 2017 Jan 20.

Preferential recognition of auto-antibodies against 4-hydroxynonenal modified DNA in the cancer patients.

Author information

1
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
2
Department of Biochemistry, King George Medical University, Lucknow, India.
3
Department of Biosciences, Integral University, Lucknow, India.

Abstract

BACKGROUND:

The structural perturbations in DNA molecule may be caused by a break in a strand, a missing base from the backbone, or a chemically changed base. These alterations in DNA that occurs naturally can result from metabolic or hydrolytic processes. DNA damage plays a major role in the mutagenesis, carcinogenesis, aging and various other patho-physiological conditions. DNA damage can be induced through hydrolysis, exposure to reactive oxygen species (ROS) and other reactive carbonyl metabolites including 4-hydroxynonenal (HNE). 4-HNE is an important lipid peroxidation product which has been implicated in the mutagenesis and carcinogenesis processes.

METHODS:

The present study examines to probe the presence of auto-antibodies against 4-hydroxynonenal damaged DNA (HNE-DNA) in various cancer subjects. In this study, the purified calf thymus DNA was damaged by the action of 4-HNE. The DNA was incubated with 4-HNE for 24 h at 37°C temperature. The binding characteristics of cancer auto-antibodies were assessed by direct binding and competitive inhibition ELISA.

RESULTS:

DNA modifications produced hyperchromicity in UV spectrum and decreased fluorescence intensity. Cancer sera exhibited enhanced binding with the 4-HNE modified calf thymus DNA as compared to its native conformer. The 4-HNE modified DNA presents unique epitopes which may be one of the factors for the auto-antibody induction in cancer patients.

CONCLUSION:

The HNE modified DNA presents unique epitopes which may be one of the factors for the autoantibody induction in cancer patients.

KEYWORDS:

DNA ; 4-hydroxynonenal (HNE); auto-antibody; cancer; glycation

PMID:
28105689
DOI:
10.1002/jcla.22130
[Indexed for MEDLINE]

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