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Int J Infect Dis. 2017 Feb;55:92-95. doi: 10.1016/j.ijid.2017.01.011. Epub 2017 Jan 16.

Diversity of New Delhi metallo-beta-lactamase-producing bacteria in China.

Author information

1
Institute for Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
2
Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
3
Center for Disease Control and Prevention of Shenyang Command, Shenyang 110034, China.
4
Department of Transfusion, General Hospital of Shenyang Military Area Command, Shenyang 110016, China.
5
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
6
The Affiliated Chenggong Hospital of Xiamen University, Xiamen 361003, China; Central Laboratory, The 174th Hospital of the Chinese People's Liberation Army, Xiamen 361003, China.
7
Institute for Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; Navy's Center for disease control and prevention, PLA, Beijing 101113, China.
8
Beijing Center for Disease Prevention and control, Beijing 100013, China.
9
Institute for Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. Electronic address: qiushf0613@hotmail.com.
10
Institute for Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. Electronic address: hongbinsong@263.net.
11
Institute for Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. Electronic address: sunys1964@hotmail.com.

Abstract

OBJECTIVES:

The prevalence and dissemination of diverse NDM-producing bacteria in China was investigated.

METHODS:

We collected 1,162 isolates from 8 cities during December 2013∼May 2015 in China. The NDM-positive strains as well as the NDM genotypes in these sample were detected via Vitek 2 compact system (bioMérieux, France), 16S rRNA gene sequencing, PCR and an S1- pulsed-field gel electrophoresis assay and Southern blot hybridization. The horizontal-transfer capability of the blaNDM gene was assessed by filter mating by using a standard E.coli J53 azide-resistant strain as the recipient.

RESULTS:

Three genotypes (NDM-1, NDM-3 and NDM-5) of NDM-producing bacteria were identified, among which the NDM-1-positive isolates were the most frequent one. For the first time, we found NDM-5-produing S.typhimurium and NDM-3-produing E.coli in China. We also found that the NDM-positive (especially NDM-3 and NDM-5) strains were completely resistant to nearly all of the antimicrobial drugs utilized and blaNDM was mostly located on diverse plasmids with sizes ranging from 30 to 670kb.

CONCLUSION:

Various species of bacteria especially the enteric pathogens with diverse NDM genotypes had spread in China. Hence, an ongoing surveillance of their dissemination is essential to prevent and control the spread of these organisms.

KEYWORDS:

China; Diversity; NDM

PMID:
28104504
DOI:
10.1016/j.ijid.2017.01.011
[Indexed for MEDLINE]
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