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Org Lett. 2017 Feb 3;19(3):496-499. doi: 10.1021/acs.orglett.6b03659. Epub 2017 Jan 19.

Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum.

Author information

1
Graduate School of Agriculture, Meiji University , 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
2
Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Chuo-ku, Chiba 260-8675, Japan.
3
Kazusa DNA Research Institute , 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.

Abstract

Ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. From the fern Lycopodium clavatum, a novel onoceroid synthase gene was cloned that showed high sequence identity with a previously identified α-onocerin synthase. Functional analysis by coexpression with pre-α-onocerin synthase in yeast led to the production of tohogenol and serratenediol. The result suggested that serratanes are directly biosynthesized from pre-α-onocerin and not from α-onocerin as previously assumed.

PMID:
28102682
DOI:
10.1021/acs.orglett.6b03659
[Indexed for MEDLINE]

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