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Phytother Res. 2017 Mar;31(3):466-474. doi: 10.1002/ptr.5772. Epub 2017 Jan 19.

Piperine, a Pungent Alkaloid from Black Pepper, Inhibits B Lymphocyte Activation and Effector Functions.

Author information

1
Department of Pathology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
2
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
3
Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.

Abstract

Piperine has several well-documented anti-inflammatory properties; however, little is known regarding its effect on humoral immunity. In this study, we describe the immunosuppressive effect of piperine on B lymphocytes, which are integral to the humoral immune response. Mouse B cells were cultured in the absence or presence of non-cytotoxic concentrations (25, 50, and 100 μM) of piperine during T-dependent or T-independent stimulation. Piperine inhibited B cell proliferation by causing G0/G1 phase cell cycle arrest in association with reduced expression of cyclin D2 and D3. The inhibitory effect of piperine was not mediated through transient receptor potential vanilloid-1 ion channel (TRPV1) because piperine also inhibited the proliferation of B cells from TRPV1-deficient mice. Expression of class II major histocompatibility complex molecules and costimulatory CD40 and CD86 on B lymphocytes was reduced in the presence of piperine, as was B cell-mediated antigen presentation to syngeneic T cells. In addition, piperine inhibited B cell synthesis of interleukin (IL)-6 and IL-10 cytokines, as well as IgM, IgG2b, and IgG3 immunoglobulins. The inhibitory effect of piperine on B lymphocyte activation and effector function warrants further investigation for possible application in the treatment of pathologies related to inappropriate humoral immune responses.

KEYWORDS:

B lymphocyte; DNA synthesis; antigen-presentation; cytokine; immunoglobulin; piperine

PMID:
28102026
DOI:
10.1002/ptr.5772
[Indexed for MEDLINE]

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