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Am J Physiol Regul Integr Comp Physiol. 2017 Apr 1;312(4):R501-R510. doi: 10.1152/ajpregu.00405.2016. Epub 2017 Jan 18.

Effects of obesity and exercise on testicular leptin signal transduction and testosterone biosynthesis in male mice.

Author information

1
Department of Kinesiology, Shenyang Sport University, Shenyang, Liaoning, China.
2
Department of Physical Education, Minnan Normal University, Zhangzhou, Fujian, China; and.
3
PE College of Beijing Normal University, Beijing, China.
4
Department of Kinesiology, Shenyang Sport University, Shenyang, Liaoning, China; changbo8387@163.com.

Abstract

To explore the role of the testicular leptin and JAK-STAT[leptin (LEP)-JAK-STAT] pathway in testosterone biosynthesis during juvenile stages and exercise for weight loss, male C57BL/6J mice were randomly divided into normal-diet and high-fat diet groups. After 10 wk, mice in the high-fat diet-fed group were further divided randomly into obese control, obese moderate-volume exercise, and obese high-volume exercise groups. Mice in the obese moderate-volume exercise group were provided with 2 h/day, 6 days/wk swimming exercise for 8 wk, and mice in the obese high-volume exercise group underwent twice the amount of daily exercise intervention as the obese moderate-volume exercise group. The results showed that a high-fat diet causes obesity, leptin resistance, inhibition of the testicular LEP-JAK-STAT pathway, decreased mRNA and protein expression of steroidogenic factor-1, steroidogenic acute regulatory protein, and the P-450 side-chain cleavage enzyme, a decrease in the serum testosterone-to-estradiol ratio, and declines in sperm quality parameters. Both moderate and high-volume exercise were able to reduce body fat and increase the mRNA and protein expression of LEP-JAK-STAT, but only moderate exercise significantly increased the mRNA and protein expression of steroidogenic factor-1, steroidogenic acute regulatory protein, and P-450 side-chain cleavage enzyme and significantly reversed the serum testosterone-to-estradiol ratio and sperm quality parameters. These findings suggest that by impairing the testicular LEP-JAK-STAT pathway, early-stage obesity inhibits the biosynthesis of testosterone and sexual development and reduces male reproductive potential. Long-term moderate and high-volume exercise can effectively reduce body fat and improve obesity-induced abnormalities in testicular leptin signal transduction, whereas only moderate-volume exercise can reverse the negative impacts of obesity on male reproductive function.

KEYWORDS:

exercise; high-fat diet-induced obesity; hypoandrogenism; leptin-Janus kinase-signal transducers and activators of transcription signaling pathway; steroidogenic enzyme

PMID:
28100475
DOI:
10.1152/ajpregu.00405.2016
[Indexed for MEDLINE]
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