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Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1021-1026. doi: 10.1073/pnas.1611191114. Epub 2017 Jan 17.

Crystal structure of Pistol, a class of self-cleaving ribozyme.

Author information

1
Department of Molecular Biochemistry and Biophysics, Yale University, New Haven, CT 06520.
2
Department of Molecular Biochemistry and Biophysics, Yale University, New Haven, CT 06520; jimin.wang@yale.edu thomas.steitz@yale.edu.
3
Howard Hughes Medical Institute, Yale University, New Haven, CT 06520.
4
Department of Chemistry, Yale University, New Haven, CT 06520-8107.

Abstract

Small self-cleaving ribozymes have been discovered in all evolutionary domains of life. They can catalyze site-specific RNA cleavage, and as a result, they have relevance in gene regulation. Comparative genomic analysis has led to the discovery of a new class of small self-cleaving ribozymes named Pistol. We report the crystal structure of Pistol at 2.97-Å resolution. Our results suggest that the Pistol ribozyme self-cleavage mechanism likely uses a guanine base in the active site pocket to carry out the phosphoester transfer reaction. The guanine G40 is in close proximity to serve as the general base for activating the nucleophile by deprotonating the 2'-hydroxyl to initiate the reaction (phosphoester transfer). Furthermore, G40 can also establish hydrogen bonding interactions with the nonbridging oxygen of the scissile phosphate. The proximity of G32 to the O5' leaving group suggests that G32 may putatively serve as the general acid. The RNA structure of Pistol also contains A-minor interactions, which seem to be important to maintain its tertiary structure and compact fold. Our findings expand the repertoire of ribozyme structures and highlight the conserved evolutionary mechanism used by ribozymes for catalysis.

KEYWORDS:

A-minor interaction; X-ray crystallography; internal transesterification; ribozyme; self-cleavage

PMID:
28096403
PMCID:
PMC5293083
DOI:
10.1073/pnas.1611191114
[Indexed for MEDLINE]
Free PMC Article

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