Format

Send to

Choose Destination
Cytometry A. 2017 Feb;91(2):180-189. doi: 10.1002/cyto.a.23054. Epub 2017 Jan 17.

High-throughput precision measurement of subcellular localization in single cells.

Author information

1
Department of Cancer Biology, Stanford University School of Medicine, Stanford, California.
2
Stanford University School of Medicine, Baxter Laboratory for Stem Cell Biology, Stanford, California.
3
Immunology and Microbial Pathogenesis, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York.
4
Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas.
5
Department of Biological Sciences, University of California Berkeley, Berkeley, California.
6
Department of Biology, Barnard College, New York, New York.
7
Developmental Biology, Stanford University School of Medicine, Stanford, California.
8
Stanford Comprehensive Cancer Institute and Department of Obstetrics and Gynecology, Stanford University, Stanford, California.
9
Department of Microbiology and Immunology, Stanford University, Stanford, California.

Abstract

To quantify visual and spatial information in single cells with a throughput of thousands of cells per second, we developed Subcellular Localization Assay (SLA). This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating to localization of proteins to and within organelles. We used SLA to detect the nuclear import of transcription factors across cell subsets in complex samples. We further measured intranuclear re-localization of target proteins across the cell cycle and upon DNA damage induction. SLA combines multiple single-cell methods to bring about a new dimension of inquiry and analysis in complex cell populations.

KEYWORDS:

DNA damage response; nuclear localization; proximity ligation assay; subcellular localization

PMID:
28094900
DOI:
10.1002/cyto.a.23054
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center