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ACS Chem Neurosci. 2017 Apr 19;8(4):884-891. doi: 10.1021/acschemneuro.6b00438. Epub 2017 Jan 30.

Bacoside-A, an Indian Traditional-Medicine Substance, Inhibits β-Amyloid Cytotoxicity, Fibrillation, and Membrane Interactions.

Author information

1
Department of Chemistry, and §Ilse Katz Institute for Nanotechnology, Ben Gurion University of the Negev , Beer Sheva 84105, Israel.
2
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, and ∥Department of Materials Science and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University , Tel Aviv 69978, Israel.

Abstract

Bacoside-A, a family of compounds extracted from the Bacopa monniera plant, is a folk-medicinal substance believed to exhibit therapeutic properties, particularly enhancing cognitive functions and improving memory. We show that bacoside-A exerted significant inhibitory effects upon cytotoxicity, fibrillation, and particularly membrane interactions of amyloid-beta (1-42) (Aβ42), the peptide playing a prominent role in Alzeheimer's disease progression and toxicity. Specifically, preincubation of bacoside-A with Aβ42 significantly reduced cell toxicity and inhibited fibril formation both in buffer solution and, more significantly, in the presence of membrane vesicles. In parallel, spectroscopic and microscopic analyses reveal that bacoside-A blocked membrane interactions of Aβ42, while formation of Aβ42 oligomers was not disrupted. These interesting phenomena suggest that inhibition of Aβ42 oligomer assembly into mature fibrils, and blocking membrane interactions of the oligomers are likely the underlying factors for ameliorating amyloid toxicity by bacoside-A and its putative physiological benefits.

KEYWORDS:

amyloid−membrane interactions; bacoside-A; fibrillation inhibitors; fluorescence anisotropy; giant vesicles; β-Amyloid; β-amyloid oligomers

PMID:
28094495
DOI:
10.1021/acschemneuro.6b00438
[Indexed for MEDLINE]

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