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Trends Genet. 2017 Feb;33(2):155-168. doi: 10.1016/j.tig.2016.12.003. Epub 2017 Jan 13.

Single-Cell Multiomics: Multiple Measurements from Single Cells.

Author information

1
Earlham Institute, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: Iain.Macaulay@earlham.ac.uk.
2
Sanger Institute - EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK; MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, UK. Electronic address: Chris.Ponting@igmm.ed.ac.uk.
3
Sanger Institute - EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK; Department of Human Genetics, University of Leuven, KU Leuven, Leuven, 3000, Belgium. Electronic address: thierry.voet@kuleuven.be.

Abstract

Single-cell sequencing provides information that is not confounded by genotypic or phenotypic heterogeneity of bulk samples. Sequencing of one molecular type (RNA, methylated DNA or open chromatin) in a single cell, furthermore, provides insights into the cell's phenotype and links to its genotype. Nevertheless, only by taking measurements of these phenotypes and genotypes from the same single cells can such inferences be made unambiguously. In this review, we survey the first experimental approaches that assay, in parallel, multiple molecular types from the same single cell, before considering the challenges and opportunities afforded by these and future technologies.

KEYWORDS:

epigenomics; genomics; multiomics; proteomics; single cell; transcriptomics

PMID:
28089370
PMCID:
PMC5303816
DOI:
10.1016/j.tig.2016.12.003
[Indexed for MEDLINE]
Free PMC Article

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