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Curr Opin Chem Biol. 2017 Feb;36:50-57. doi: 10.1016/j.cbpa.2016.12.026. Epub 2017 Jan 12.

Cell-selective proteomics for biological discovery.

Author information

1
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
2
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States. Electronic address: tirrell@caltech.edu.

Abstract

Cells alter the proteome to respond to environmental and developmental cues. Global analysis of proteomic responses is of limited value in heterogeneous environments, where there is no 'average' cell. Advances in sequencing, protein labeling, mass spectrometry, and data analysis have fueled recent progress in the investigation of specific subpopulations of cells in complex systems. Here we highlight recently developed chemical tools that enable cell-selective proteomic analysis of complex biological systems, from bacterial pathogens to whole animals.

PMID:
28088696
PMCID:
PMC5337159
DOI:
10.1016/j.cbpa.2016.12.026
[Indexed for MEDLINE]
Free PMC Article

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