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BMC Complement Altern Med. 2017 Jan 14;17(1):47. doi: 10.1186/s12906-017-1555-0.

Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum.

Author information

1
Department of Physiology, Faculty of Medicine, Malaysia Institute of Medical Research, Kuala Lumpur, 50603, Malaysia.
2
Department of Physiology, Faculty of Medicine, Malaysia Institute of Medical Research, Kuala Lumpur, 50603, Malaysia. kimkh@um.edu.my.
3
Malaysia Institute of Medical Research, Kuala Lumpur, 50603, Malaysia.
4
University College Lincoln, Petaling Jaya, 47301, Malaysia.

Abstract

BACKGROUND:

Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammation give rise to using medicinal herbs as alternative therapies. This study aimed to evaluate the anti-inflammatory effect of isolated compounds from the ethyl acetate (EtOAc) fraction of P. granatum by determination of their inhibitory effects on lipopolysaccharide (LPS), stimulated nitric oxide (NO), prostaglandin E2 (PGE-2), interleukin-6 (IL-6) and cyclooxxgenase-2 (COX-2) release from RAW264.7 cells.

METHODS:

The compounds ellagic acid, gallic acid and punicalagin A&B were isolated from EtOAc by high performance liquid chromatography (HPLC) and further identified by mass spectrometry (MS). The inhibitory effect of ellagic acid, gallic acid and punicalagin A&B were evaluated on the production of LPS-induced NO by Griess reagent, PGE-2 and IL-6 by immunoassay kit and prostaglandin E2 competitive ELISA kit, and COX-2 by Western blotting.

RESULTS:

Ellagic acid, gallic acid and punicalagin A&B potentially inhibited LPS-induced NO, PGE-2 and IL-6 production.

CONCLUSION:

The results indicate that ellagic acid, gallic acid and punicalagin may be the compounds responsible for the anti-inflammatory potential of P. granatum.

KEYWORDS:

Cytokines; Cytotoxicity; Ellagic acid; Gallic acid; Inflammation; Punica granatum; Punicalagin

PMID:
28088220
PMCID:
PMC5237561
DOI:
10.1186/s12906-017-1555-0
[Indexed for MEDLINE]
Free PMC Article

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