Format

Send to

Choose Destination
Development. 2017 Feb 15;144(4):677-686. doi: 10.1242/dev.146076. Epub 2017 Jan 13.

Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation.

Author information

1
Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Patterson Labs 532, 2401 Speedway, Austin, TX 78712, USA.
2
Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Patterson Labs 532, 2401 Speedway, Austin, TX 78712, USA d.stein@mail.utexas.edu.

Abstract

Formation of the Drosophila embryonic termini is controlled by the localized activation of the receptor tyrosine kinase Torso. Both Torso and Torso's presumed ligand, Trunk, are expressed uniformly in the early embryo. Polar activation of Torso requires Torso-like, which is expressed by follicle cells adjacent to the ends of the developing oocyte. We find that Torso expressed at high levels in cultured Drosophila cells is activated by individual application of Trunk, Torso-like or another known Torso ligand, Prothoracicotropic Hormone. In addition to assays of downstream signaling activity, Torso dimerization was detected using bimolecular fluorescence complementation. Trunk and Torso-like were active when co-transfected with Torso and when presented to Torso-expressing cells in conditioned medium. Trunk and Torso-like were also taken up from conditioned medium specifically by cells expressing Torso. At low levels of Torso, similar to those present in the embryo, Trunk and Torso-like alone were ineffective but acted synergistically to stimulate Torso signaling. Our results suggest that Torso interacts with both Trunk and Torso-like, which cooperate to mediate dimerization and activation of Torso at the ends of the Drosophila embryo.

KEYWORDS:

Drosophila; MACPF; Membrane attack complex perforin; RTK; Receptor tyrosine kinase; Terminal

PMID:
28087630
PMCID:
PMC5312039
DOI:
10.1242/dev.146076
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center