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J Nanobiotechnology. 2017 Jan 13;15(1):8. doi: 10.1186/s12951-016-0246-1.

C60 fullerene as promising therapeutic agent for correcting and preventing skeletal muscle fatigue.

Author information

1
Department of Biophysics, Taras Shevchenko National University of Kyiv, Volodymyrska Str. 60, Kiev, 01601, Ukraine.
2
Department of Movement Physiology, Bogomoletz Institute of Physiology, Bogomoletz Str. 4, Kiev, 01024, Ukraine.
3
Department of Movement Physiology, Bogomoletz Institute of Physiology, Bogomoletz Str. 4, Kiev, 01024, Ukraine. maznychenko@biph.kiev.ua.
4
Department of Hypoxic States Investigation, Bogomoletz Institute of Physiology, Bogomoletz Str. 4, Kiev, 01024, Ukraine.
5
Joint Institute for Nuclear Research, Joliot-Curie Str. 6, Dubna, Moscow Region, Russia.
6
Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Weimarer Str. 25, 98693, Ilmenau, Germany.
7
University of Physical Education and Sport, Kazimierza Górskiego Str.1, 80-336, Gdansk, Poland.
8
Lesia Ukrainka Eastern European National University, Volya Avenue 13, Lutsk, 43025, Ukraine.

Abstract

BACKGROUND:

Bioactive soluble carbon nanostructures, such as the C60 fullerene can bond with up to six electrons, thus serving by a powerful scavenger of reactive oxygen species similarly to many natural antioxidants, widely used to decrease the muscle fatigue effects. The aim of the study is to define action of the pristine C60 fullerene aqueous colloid solution (C60FAS), on the post-fatigue recovering of m. triceps surae in anaesthetized rats.

RESULTS:

During fatigue development, we observed decrease in the muscle effort level before C60FAS administration. After the application of C60FAS, a slower effort decrease, followed by the prolonged retention of a certain level, was recorded. An analysis of the metabolic process changes accompanying muscle fatigue showed an increase in the oxidative stress markers H 2 O 2 (hydrogen peroxide) and TBARS (thiobarbituric acid reactive substances) in relation to the intact muscles. After C60FAS administration, the TBARS content and H 2 O 2 level were decreased. The endogenous antioxidant system demonstrated a similar effect because the GSH (reduced glutathione) in the muscles and the CAT (catalase) enzyme activity were increased during fatigue.

CONCLUSIONS:

C60FAS leads to reduction in the recovery time of the muscle contraction force and to increase in the time of active muscle functioning before appearance of steady fatigue effects. Therefore, it is possible that C60FAS affects the prooxidant-antioxidant muscle tissue homeostasis, subsequently increasing muscle endurance.

KEYWORDS:

Antioxidant system; C60 fullerene; Electrical stimulation; Oxidative stress markers; Skeletal muscles fatigue

PMID:
28086894
PMCID:
PMC5237293
DOI:
10.1186/s12951-016-0246-1
[Indexed for MEDLINE]
Free PMC Article

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