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PLoS One. 2017 Jan 13;12(1):e0168964. doi: 10.1371/journal.pone.0168964. eCollection 2017.

Levo-Tetrahydroberberrubine Produces Anxiolytic-Like Effects in Mice through the 5-HT1A Receptor.

Author information

1
Beijing Institute of Basic Medical Sciences, Haidian District, Beijing, China.
2
Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China.
3
The 89 Hospital of PLA, WeiFang, China.

Abstract

Tetrahydroprotoberberines (THPBs) are isoquinoline alkaloids isolated from the Chinese herb Corydalis yanhusuo. In the present study, we performed competitive binding assays to examine the binding of l-THBr to neurotransmitter receptors known to be involved in sedation, hypnosis and anxiety. Our results show that l-THBr does not interact with GABAergic receptors but has binding affinities for dopamine and serotonin receptors. In addition, cAMP and [35S]GTPγS assays were used to determine the agonist or antagonist properties of l-THBr at dopamine (D1, D2) or serotonin (5-HT) receptors. Our results show that l-THBr displays D1 and D2 antagonist and 5-HT1A agonist properties. Moreover, l-THBr-treated rodents exhibit anxiolytic-like effects in the light/dark box and elevated plus-maze tests, and the anxiolytic effect of l-THBr can be reduced by WAY-100635, a selective 5-HT1A receptor antagonist. Our results suggest that l-THBr may produce potent anxiolytic-like effects mainly through serotonin receptors.

PMID:
28085967
PMCID:
PMC5234788
DOI:
10.1371/journal.pone.0168964
[Indexed for MEDLINE]
Free PMC Article

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