High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Cav3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells

Kazuki Fukami; Erina Asano; Mai Ueda; Fumiko Sekiguchi; Shigeru Yoshida; Atsufumi Kawabata

doi:10.1016/j.jphs.2016.12.004

High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Ca_v3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells

J Pharmacol Sci. 2017 Jan;133(1):57-60. doi: 10.1016/j.jphs.2016.12.004. Epub 2017 Jan 3.

Authors

Kazuki Fukami¹, Erina Asano¹, Mai Ueda¹, Fumiko Sekiguchi¹, Shigeru Yoshida², Atsufumi Kawabata³

Affiliations

¹ Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan.
² Department of Life Science, Faculty of Science and Engineering, Kindai University, Higashi-Osaka 577-8502, Japan.
³ Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-Osaka 577-8502, Japan. Electronic address: kawabata@phar.kindai.ac.jp.

PMID: 28081948
DOI: 10.1016/j.jphs.2016.12.004

Abstract

Given that Ca_v3.2 T-type Ca²⁺ channels were functionally regulated by asparagine (N)-linked glycosylation, we examined effects of high glucose on the function of Ca_v3.2, known to regulate secretory function, in neuroendocrine-like differentiated prostate cancer LNCaP cells. High glucose accelerated the increased channel function and overexpression of Ca_v3.2 during neuroendocrine differentiation, the former prevented by enzymatic inhibition of N-glycosylation and cleavage of N-glycans. Hyperglycemia thus appears to induce N-linked glycosylation-mediated functional upregulation and overexpression of Ca_v3.2 in neuroendocrine-like differentiated prostate cancer cells.

Keywords: Asparagine-linked glycosylation; Ca(v)3.2 T-type calcium channel; Prostate cancer.

MeSH terms

1-Methyl-3-isobutylxanthine / pharmacology
Bucladesine / pharmacology
Calcium Channels, T-Type / biosynthesis*
Cell Differentiation*
Cell Line, Tumor
Gene Expression Regulation, Neoplastic*
Glucose / pharmacology*
Glycosylation / drug effects
Humans
Male
Membrane Potentials / drug effects
Prostatic Neoplasms / metabolism*
Prostatic Neoplasms / pathology*
Tunicamycin / pharmacology
Up-Regulation / drug effects*

Substances

CACNA1H protein, human
Calcium Channels, T-Type
Tunicamycin
Bucladesine
Glucose
1-Methyl-3-isobutylxanthine