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Am J Chin Med. 2017;45(1):53-66. doi: 10.1142/S0192415X17500057. Epub 2017 Jan 13.

Punicalagin Pretreatment Attenuates Myocardial Ischemia-Reperfusion Injury via Activation of AMPK.

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* Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
§ Department of Geriatrics, Xi'an Central Hospital, Xi'an, P.R. China.
‡ Department of Cardiology, Traditional Chinese Medicine Hospital of Baoji City, Baoji, P.R. China.
¶ Department of Cardiology, Shengli Oilfield Central Hospital, Dongying, P.R. China.
† Department of Physiology, Fourth Military Medical University, Xi'an, P.R. China.


Punicalagin (PUN), a major bioactive component in pomegranate juice, has been proven to exert neuroprotective effects against cerebral ischemia/reperfusion (I/R) insult via anti-oxidant properties. This study aims to investigate whether PUN provides cardioprotection against myocardial I/R (MI/R) injury and the underlying mechanisms. PUN (30[Formula: see text]mg/kg/d) or vehicle was intragastrically administered to Sprague-Dawley rats for one week before the operation. MI/R was induced by ligating the left anterior descending coronary artery for 30[Formula: see text]min and subsequent reperfusion for 3[Formula: see text]h. PUN pretreatment conferred cardioprotective effects against MI/R injury by improving cardiac function, limiting infarct size, reducing serum creatine kinase-MB and lactate dehydrogenase activities, and suppressing cardiomyocyte apoptosis. Moreover, PUN pretreatment inhibited I/R-induced myocardial oxidative stress as evidenced by decreased generation of superoxide content and malonaldialdehyde formation and increased antioxidant capability. Furthermore, PUN pretreatment increased adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation in I/R hearts. AMPK inhibitor compound c inhibited PUN-enhanced AMPK phosphorylation, and blunted PUN-mediated anti-oxidative effects and cardioprotection. These results indicate for the first time that PUN pretreatment protect against I/R-induced oxidative stress and myocardial injury via activation of AMPK.


AMPK; Myocardial Ischemia/Reperfusion; Oxidative Stress; Punicalagin

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