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Cell Host Microbe. 2017 Jan 11;21(1):35-46. doi: 10.1016/j.chom.2016.12.010.

Mapping and Role of the CD8+ T Cell Response During Primary Zika Virus Infection in Mice.

Author information

1
Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.
2
Department of Medicine, Molecular Microbiology, Pathology, and Immunology, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA.
3
Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA. Electronic address: sujan@lji.org.

Abstract

CD8+ T cells may play a dual role in protection against and pathogenesis of flaviviruses, including Zika virus (ZIKV). We evaluated the CD8+ T cell response in ZIKV-infected LysMCre+IFNARfl/fl C57BL/6 (H-2b) mice lacking the type I interferon receptor in a subset of myeloid cells. In total, 26 and 15 CD8+ T cell-reactive peptides for ZIKV African (MR766) and Asian (FSS13025) lineage strains, respectively, were identified and validated. CD8+ T cells from infected mice were polyfunctional and mediated cytotoxicity. Adoptive transfer of ZIKV-immune CD8+ T cells reduced viral burdens, whereas their depletion led to higher tissue burdens, and CD8-/- mice displayed higher mortality with ZIKV infection. Collectively, these results demonstrate that CD8+ T cells protect against ZIKV infection. Further, this study provides a T cell competent mouse model for investigating ZIKV-specific T cell responses.

KEYWORDS:

CD8(+) T cell; LysMCre(+)IFNAR(fl/fl); Zika virus; epitope; mouse model; peptide

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PMID:
28081442
PMCID:
PMC5234855
DOI:
10.1016/j.chom.2016.12.010
[Indexed for MEDLINE]
Free PMC Article

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