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Exp Lung Res. 2017 Feb;43(1):1-7. doi: 10.1080/01902148.2016.1267823. Epub 2017 Jan 12.

Low concentration of oleic acid exacerbates LPS-induced cell death and inflammation in human alveolar epithelial cells.

Author information

1
a Department of Biochemistry , Veterinary Faculty, Mustafa Kemal University , Hatay , Turkey.
2
b Department of Medical Biology , Faculty of Medicine, Trakya University , Edirne , Turkey.

Abstract

PURPOSE:

The current study aimed to investigate in vitro effects of oleic acid on lipopolysaccharide (LPS)-induced acute lung injury in the human lung epithelial cells (A549).

MATERIALS AND METHODS:

The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests. Selected gene expression levels were analyzed by Real-Time Quantitative-Polymerase Chain Reaction (RT-qPCR).

RESULTS:

24 hours of LPS (100 ng/mL) exposure decreased the cells' viability by 44.6% compared to untreated control. Low concentration (2.5 nM) of oleic acid slightly suppressed the cell survival by 9.1% analyzed 24 hours after incubation. However, oleic acid pretreatment before LPS exposure significantly increased cell survival loss to 63.9%. LPS exposure decreased the expressions of catalase (CAT) and glutathione peroxidase (GPx) mRNA levels by 2.8 and 2.5 fold, respectively. Moreover, pretreatment of the cells with oleic acid strengthened LPS-decreased expressions of CAT and GPx genes by 3.5 and 6.7 fold, respectively. The mRNA expressions of superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), interleukin-1β, IL-12, COX-2, caspase-3 and caspase-8 were increased by 2.4, 2.2, 2.2, 2.3, 3.0, 2.6, and 2.5 fold, respectively, by LPS, and oleic acid pretreatment significantly potentiated the effect of LPS.

CONCLUSION:

Oleic acid worsens LPS-induced cell death by potentiating oxidative stress and inflammation in A549 lung epithelial cells.

KEYWORDS:

apoptosis; inflammation; lipopolysaccharide; lung injury; oleic acid

PMID:
28080141
DOI:
10.1080/01902148.2016.1267823
[Indexed for MEDLINE]

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