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Molecules. 2017 Jan 10;22(1). pii: E107. doi: 10.3390/molecules22010107.

Inhibitory Effects of Probiotic Lactobacillus on the Growth of Human Colonic Carcinoma Cell Line HT-29.

Author information

1
Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan. g1010046@pu.edu.tw.
2
Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan. ymhsieh@pu.edu.tw.
3
New Bellus Enterprises Co., Ltd. No. 48, Industrial Rd., Erh Chen Vil., Kuan Tien Dist., Tainan City 72042, Taiwan. john@newbellus.com.tw.
4
Department of Food Science and Technology, Hungkuang University, Taichung City 43302, Taiwan. tsaicc@sunrise.hk.edu.tw.

Abstract

This study was conducted to investigate the inhibitory effect of Lactobacillus cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1.19, which showed a significant difference from the other control and LAB groups (p < 0.05), which therefore led to the inference that the BCRC17010 strain exerts a pro-apoptotic effect on the HT-29 cells. Upon co-culture with HT-29 cells for 4, 8 and 12 h, the BCRC14625 strain (10⁸ cfu/mL) demonstrated a significant increase in lactate dehydrogenase (LDH) activity (p < 0.05), causing harm to the HT-29 cell membrane; further, after an 8-h co-culture with the HT-29 cells, it induced the secretion of nitric oxide (NO) from the HT-29 cells. Some lactic acid bacteria (LAB) strains have ability to inhibit the growth of the colorectal cancer cell line HT-29 Bax/Bcl-2 pathway or NO production. In summary, we demonstrated that the BCRC17010 strain, good abilities of adhesion and increased LDH release, was the best probiotic potential for inhibition of HT-29 growth amongst the seven LAB strains tested in vitro.

KEYWORDS:

Bax/Bcl-2 ratio; HT-29 cells; Lactobacillus; interleukin-8; lactate dehydrogenase

PMID:
28075415
PMCID:
PMC6155858
DOI:
10.3390/molecules22010107
[Indexed for MEDLINE]
Free PMC Article

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