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Emerg Infect Dis. 2017 Apr;23(4):680-681. doi: 10.3201/eid2304.161772. Epub 2017 Apr 15.

Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay.

Abstract

We assayed Zika virus-infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days.

KEYWORDS:

Culicidae; Vero cells; Zika virus; arboviruses; cold chain; mosquitoes; plaque assay; real-time reverse transcription PCR; virus RNA; virus detection; virus persistence in mosquitoes; viruses

PMID:
28075325
PMCID:
PMC5367413
DOI:
10.3201/eid2304.161772
[Indexed for MEDLINE]
Free PMC Article

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