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Yeast. 2017 May;34(5):205-221. doi: 10.1002/yea.3228. Epub 2017 Feb 21.

Saccharomyces cerevisiae Shuttle vectors.

Author information

1
D-BSSE, ETH Zurich and Swiss Institute of Bioinformatics, Zurich, Switzerland.
2
Life Science Zurich PhD Program on Molecular and Translational Biomedicine, Zurich, Switzerland.
3
Competence Centre for Personalized Medicine, Zurich, Switzerland.

Abstract

Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties.

KEYWORDS:

Saccharomyces cerevisiae; copy number; genomic integration; plasmid loss; plasmids; shuttle vectors

PMID:
28072905
DOI:
10.1002/yea.3228
[Indexed for MEDLINE]
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