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J Food Sci. 2017 Feb;82(2):545-552. doi: 10.1111/1750-3841.13620. Epub 2017 Jan 10.

Effects of Catechol O-Methyl Transferase Inhibition on Anti-Inflammatory Activity of Luteolin Metabolites.

Author information

1
Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Inst., Gyeonggi, 463-746, Republic of Korea.

Abstract

Although luteolin is known to have potent anti-inflammatory activities, much less information has been provided on such activities of its hepatic metabolites. Luteolin was subjected to hepatic metabolism in HepG2 cells either without or with catechol O-methyl transferase (COMT) inhibitor. To identify hepatic metabolites of luteolin without (luteolin metabolites, LMs) or with COMT inhibitor (LMs+CI), metabolites were treated by β-glucuronidase and sulfatase, and found that they were composed of glucuronide and sulfate conjugates of diosmetin in LMs or these conjugates of luteolin in LMs+CI. LMs and LMs+CI were examined for their anti-inflammatory activities on LPS stimulated Raw 264.7 cells. Expression of iNOS and production of nitric oxide and pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 were suppressed more effectively by the treatment with LMs+CI than LMs. Our data provide a new insight on possible improvement in functional properties of luteolin on target cells by modifying their metabolic pathway in hepatocytes.

KEYWORDS:

catechol O-methyl transferase; inflammation; luteolin; metabolites

PMID:
28071803
DOI:
10.1111/1750-3841.13620
[Indexed for MEDLINE]

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