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PLoS Genet. 2017 Jan 9;13(1):e1006541. doi: 10.1371/journal.pgen.1006541. eCollection 2017 Jan.

Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes.

Author information

1
U1038, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble (BIG), Grenoble, France.
2
Laboratoire Biologie à Grande Echelle (BGE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), Grenoble, France.
3
U1038, Institut national de la santé et de la recherche médicale (Inserm), Grenoble, France.
4
Centre National de la Recherche Scientifique (CNRS), Institut de Biosciences et Biotechnologies de Grenoble (BIG), Grenoble, France.
5
Centre National de Génotypage, Institut de Génomique, Commissariat à l'énergie atomique et aux énergies alternatives, Evry, France.

Abstract

Bromodomain and Extra-terminal motif (BET) proteins play a central role in transcription regulation and chromatin signalling pathways. They are present in unicellular eukaryotes and in this study, the role of the BET protein Bdf1 has been explored in Saccharomyces cerevisiae. Mutation of Bdf1 bromodomains revealed defects on both the formation of spores and the meiotic progression, blocking cells at the exit from prophase, before the first meiotic division. This phenotype is associated with a massive deregulation of the transcription of meiotic genes and Bdf1 bromodomains are required for appropriate expression of the key meiotic transcription factor NDT80 and almost all the Ndt80-inducible genes, including APC complex components. Bdf1 notably accumulates on the promoter of Ndt80 and its recruitment is dependent on Bdf1 bromodomains. In addition, the ectopic expression of NDT80 during meiosis partially bypasses this dependency. Finally, purification of Bdf1 partners identified two independent complexes with Bdf2 or the SWR complex, neither of which was required to complete sporulation. Taken together, our results unveil a new role for Bdf1 -working independently from its predominant protein partners Bdf2 and the SWR1 complex-as a regulator of meiosis-specific genes.

PMID:
28068333
PMCID:
PMC5261807
DOI:
10.1371/journal.pgen.1006541
[Indexed for MEDLINE]
Free PMC Article

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