Format

Send to

Choose Destination
Cell Biol Int. 2017 Mar;41(3):320-327. doi: 10.1002/cbin.10728. Epub 2017 Jan 23.

The effect of glutamate on ghrelin release in mice.

Author information

1
Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
2
Departments of Internal Medicine (Divisions of Hypothalamic Research and Endocrinology & Metabolism) and Psychiatry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9077, USA.
3
Area of Life-NanoBio, Division of Strategy, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.

Abstract

Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

KEYWORDS:

CREB2; GPRC5B; extracellular signal-regulated kinase (ERK); ghrelin; glutamate

PMID:
28067437
DOI:
10.1002/cbin.10728
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center