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Methods Cell Biol. 2017;137:225-238. doi: 10.1016/bs.mcb.2016.03.029. Epub 2016 Jun 28.

Analysis of cytokinesis by electron microscopy.

Author information

1
Technische Universität Dresden, Dresden, Germany.
2
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Abstract

Following up on a chapter on the Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis (MCB 79, 101-119), we present an adaptation of our established protocol for the ultrastructural analysis of either permeabilized or injected embryonic systems. We prepared both drug-treated early C. elegans embryos and fluorescently labeled sea urchin embryos of Lytechinus pictus for ultrastructural studies on animal cytokinesis. Here we focus on the initial preparation steps of postmitotic embryos for high-pressure freezing and subsequent electron microscopy with an emphasis on electron tomography. The advantages and limitations of our extended protocol will be discussed.

KEYWORDS:

Abscission; Correlative light electron microscopy; Cytokinesis; Electron microscopy; Electron tomography; High-pressure freezing

PMID:
28065307
DOI:
10.1016/bs.mcb.2016.03.029
[Indexed for MEDLINE]

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