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Bioorg Med Chem. 2017 Feb 1;25(3):1250-1259. doi: 10.1016/j.bmc.2016.12.039. Epub 2016 Dec 27.

New N- and O-arylpiperazinylalkyl pyrimidines and 2-methylquinazolines derivatives as 5-HT7 and 5-HT1A receptor ligands: Synthesis, structure-activity relationships, and molecular modeling studies.

Author information

1
Dipartimento di Scienze del Farmaco, UniversitĂ  di Catania, viale A. Doria 6, Catania 95125, Italy. Electronic address: s.intagliata@studium.unict.it.
2
Dipartimento di Scienze del Farmaco, UniversitĂ  di Catania, viale A. Doria 6, Catania 95125, Italy.
3
IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri", via La Masa 19, Milano 20156, Italy.
4
Department of Medicinal Chemistry, Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, 31-343 Krakow, Poland.

Abstract

Based on our earlier studies of structure activity relationships on 4-substituted piperazine derivatives, in this work we synthesized a novel set of long-chain arylpiperazines with the purpose of elucidating if some structural modifications in the terminal fragment could affect the binding affinity for the 5-HT7 and 5-HT1A receptors. In this new series, the quinazolinone system of the previous derivatives was replaced by a 6-phenylpyrimidine or a 2-methylquinazoline, which were used as versatile building blocks for the preparation of new compounds. A 4-arylpiperazine moiety through a five methylene chain was anchored at the nitrogen or oxygen atom of the heterocyclic scaffolds. The substituents borne by the piperazine nucleus were phenyl, phenylmethyl, 3- or 4-chlorophenyl, and 2-ethoxyphenyl. Binding tests, performed on human cloned 5-HT7 and 5-HT1A receptors, showed that, among the newly synthesized derivatives, 4-[5-[4-(2-ethoxyphenyl)-1-piperazinyl]pentoxy]-6-phenyl-pyrimidine (13) and 3-[5-[4-(2-ethoxyphenyl)-1-piperazinyl]pentyl]-2-methyl-4(3H)-quinazolinone (20) displayed the best affinity values, Ki=23.5 and 8.42nM for 5-HT7 and 6.96 and 2.99nM for 5-HT1A receptors, respectively. Moreover, the functional properties for both compounds were further evaluated using the cAMP assay. Finally, a molecular modeling study has been performed for 5-HT7 and 5-HT1A receptor homology models to investigate the binding mode of N- and O-alkylated pyrimidinones/pyrimidines 4-13, 2-methylquinazolinones/quinazolines 17-22, and previously reported 2- and 3-substituted quinazolinones 23-30.

KEYWORDS:

5-HT(1A) receptor ligands; 5-HT(7) receptor ligands; Long-chain arylpiperazines; Molecular modeling

PMID:
28063784
DOI:
10.1016/j.bmc.2016.12.039
[Indexed for MEDLINE]

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