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Biosens Bioelectron. 2017 May 15;91:431-435. doi: 10.1016/j.bios.2016.12.058. Epub 2016 Dec 30.

Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein.

Author information

1
Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, PR China.
2
Departments of Histology and Embryology, Basic Medical College, Jilin University, Changchun 130021, PR China.
3
Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China. Electronic address: chenyp@nanoctr.cn.
4
Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, PR China. Electronic address: yangl330@nenu.edu.cn.

Abstract

We have developed a sensitive photonic crystal fiber (PCF)-based immunosensor for detection of alpha fetoprotein (AFP). The unique PCF possesses a morphology characterized by numerous pore structures and a large surface area-to-volume ratio, which can be used as an immune-reaction carrier to improve the sensitivity and reaction speed of AFP detection. The PCF-based immunosensor possesses a low limit of detection of 0.1ng/mL, which is five times lower than that of the capillary-based sensor and 35 times lower than that of the traditional enzyme-linked immunosorbent assay. The wide linear dynamic range of 0.1-150ng/mL makes the developed immunosensor suitable for clinical practice. The proposed method was successfully applied to AFP detection in a clinical serum sample with acceptable precision. It is indicated that the present PCF-based immunosensor could be used as an attractive analytical platform for sensitive and specific detection of cancer biomarkers.

KEYWORDS:

Cancer biomarker detection; Immunosensor; Photonic crystal fiber

PMID:
28063391
DOI:
10.1016/j.bios.2016.12.058
[Indexed for MEDLINE]

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