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Methods Mol Biol. 2017;1559:343-365. doi: 10.1007/978-1-4939-6786-5_24.

Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools.

Author information

1
Clinical Neurosciences, Faculty of Medicine, University of Southampton, Southampton General Hospital, Tremona Road, Southampton, SO16 6YD, UK.
2
Department of Life, Health and Chemical Sciences, Open University, Milton Keynes, UK.
3
Clinical Neurosciences, Faculty of Medicine, University of Southampton, Southampton General Hospital, Tremona Road, Southampton, SO16 6YD, UK. rcn@soton.ac.uk.

Abstract

In this chapter we describe in detail the surgical and imaging techniques employed for the study of the anatomical routes of drainage of cerebrospinal fluid (CSF) and interstitial fluid (ISF) from the brain. The types of tracers, sites of injection, and volumes injected are crucial. For example, when testing the drainage of ISF from the parenchyma, volumes larger than 0.5 μL result in spillage of ISF into the ventricular CSF.

KEYWORDS:

Alzheimer’s disease; Cerebrospinal fluid; Cisterna magna injections; Interstitial fluid; Intraparenchymal stereotactic injections; Mouse brain; Perivascular lymphatic drainage; Rodent perfusion; Solute clearance

PMID:
28063056
DOI:
10.1007/978-1-4939-6786-5_24
[Indexed for MEDLINE]

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