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Biotechnol Bioeng. 2017 Jun;114(6):1331-1342. doi: 10.1002/bit.26244. Epub 2017 Feb 2.

Computational de novo design of antibodies binding to a peptide with high affinity.

Author information

1
Department of Chemical Engineering, University Park, Pennsylvania, 16802.
2
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, 16802.
3
Department of Physics and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801.

Abstract

Antibody drugs play a critical role in infectious diseases, cancer, autoimmune diseases, and inflammation. However, experimental methods for the generation of therapeutic antibodies such as using immunized mice or directed evolution remain time consuming and cannot target a specific antigen epitope. Here, we describe the application of a computational framework called OptMAVEn combined with molecular dynamics to de novo design antibodies. Our reference system is antibody 2D10, a single-chain antibody (scFv) that recognizes the dodecapeptide DVFYPYPYASGS, a peptide mimic of mannose-containing carbohydrates. Five de novo designed scFvs sharing less than 75% sequence similarity to all existing natural antibody sequences were generated using OptMAVEn and their binding to the dodecapeptide was experimentally characterized by biolayer interferometry and isothermal titration calorimetry. Among them, three scFvs show binding affinity to the dodecapeptide at the nM level. Critically, these de novo designed scFvs exhibit considerably diverse modeled binding modes with the dodecapeptide. The results demonstrate the potential of OptMAVEn for the de novo design of thermally and conformationally stable antibodies with high binding affinity to antigens and encourage the targeting of other antigen targets in the future. Biotechnol. Bioeng. 2017;114: 1331-1342.

KEYWORDS:

OptMAVEn; antibody structure prediction; biolayer interferometry; computational antibody design; de novo design; single-chain antibodies

PMID:
28059445
PMCID:
PMC5726764
DOI:
10.1002/bit.26244
[Indexed for MEDLINE]
Free PMC Article

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