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Med Oncol. 2017 Feb;34(2):22. doi: 10.1007/s12032-016-0877-y. Epub 2017 Jan 5.

A surface tension magnetophoretic device for rare cell isolation and characterization.

Author information

1
Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
2
Department of Urology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
3
Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA. dss@jhu.edu.

Abstract

The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a 'real-time liquid biopsy'. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive 'lab on a chip' device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

KEYWORDS:

Circulating tumor cells; Magnetophoresis; Micro-fluidic device; Prostate cancer; Rare cells

PMID:
28058627
PMCID:
PMC5801755
DOI:
10.1007/s12032-016-0877-y
[Indexed for MEDLINE]
Free PMC Article

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