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Appl Microbiol Biotechnol. 2017 Feb;101(3):1227-1237. doi: 10.1007/s00253-016-8073-8. Epub 2017 Jan 5.

Comparative genomic analysis of bacteriocin-producing Weissella cibaria 110.

Author information

1
Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan.
2
Bioinformatics Program, Taiwan International Graduate Program, Institute of Information Science, Academia Sinica, Taipei, 11529, Taiwan.
3
Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd, GuiShan Township, Taoyuan County, 333, Taiwan.
4
Faculty of Agro-Industry, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat Campus, Thung Yai, 80240, Thailand.
5
Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan. cchang@ym.edu.tw.
6
Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei, 11221, Taiwan. cchang@ym.edu.tw.

Abstract

Weissella cibaria 110 was isolated from plaa-som, a Thai fermented fish product, and known to produce the weissellicin 110 bacteriocin. We carried out comprehensive comparative genomic analysis of W. cibaria 110 with four other non-bacteriocin-producing W. cibaria strains and identified potential antibiotic-resistant genes. We further identified a type III restriction-modification system, a TA system, and a bacteriocin gene cluster that are unique in W. cibaria 110. Genes related to bacteriocin biosynthesis are organized in clusters and are encoded with minimum genetic machinery consisting of structural cognate immunity genes, including ABC transporter and immunity protein. Finally, we predicted W. cibaria 110 to produce a class IId bacteriocin, weissellicin 110, which is 31 amino acids in length and contains a 21-amino-acid N-terminal leader peptide. This is the first bacteriocin-producing sequencing genome in W. cibaria, and we describe the difference between the bacteriocin-producing and non bacteriocin-producing strains from genome point of view.

KEYWORDS:

Bacteriocin; Draft genome sequence; Probiotics; Weissella cibaria

PMID:
28058448
DOI:
10.1007/s00253-016-8073-8
[Indexed for MEDLINE]

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