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J Vet Sci. 2018 Jan 31;19(1):35-43. doi: 10.4142/jvs.2018.19.1.35.

Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene.

Author information

1
Indian Council of Agricultural Research-Indian Veterinary Research Institute, Uttar Pradesh 243122, India.
2
Department of Biomedical Sciences, One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, Federation of Saint Kitts and Nevis.
3
Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
4
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest 1143, Hungary.

Abstract

Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All nsp4 genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that nsp4 genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 nsp4 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.

KEYWORDS:

enterotoxins; nsp4 gene evolution; porcine; rotavirus; viral nonstructural proteins

PMID:
28057906
PMCID:
PMC5799398
DOI:
10.4142/jvs.2018.19.1.35
[Indexed for MEDLINE]
Free PMC Article

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