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J Biol Chem. 2017 Feb 24;292(8):3252-3261. doi: 10.1074/jbc.M116.765081. Epub 2017 Jan 5.

Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex.

Author information

1
From the Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
2
the Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France, and.
3
the Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
4
From the Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France, cascales@imm.cnrs.fr.

Abstract

The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in Flavobacterium, S-layer components in Tannerella forsythia, and tooth tissue-degrading enzymes in the oral pathogen Porphyromonas gingivalis Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.

KEYWORDS:

bacterial pathogenesis; membrane protein; microbiology; protein assembly; protein complex; protein purification; protein secretion; secretion

PMID:
28057754
PMCID:
PMC5336160
DOI:
10.1074/jbc.M116.765081
[Indexed for MEDLINE]
Free PMC Article

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