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Biomarkers. 2017 May - Jun;22(3-4):372-382. doi: 10.1080/1354750X.2017.1279215. Epub 2017 Jan 25.

Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.

Author information

1
a Department of Chemistry and Biochemistry , University of Toledo , Toledo , OH , USA.
2
b Department of Neuroscience , University of Toledo , Toledo , OH , USA.
3
c Department of Mathematics and Statistics , University of Toledo , Toledo , OH , USA.
4
d Department of Pathology , University of Toledo, Toledo , OH, USA.
5
e Department of Emergency Medicine , University of Toledo , Toledo , OH , USA.

Abstract

CONTEXT:

Quantitative changes of salivary proteins due to acute stress were detected.

OBJECTIVE:

To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.

MATERIALS AND METHODS:

Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.

RESULTS:

Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.

DISCUSSION AND CONCLUSION:

Alpha-amylase and cystatins are potential salivary markers of acute stress, but further validation should be performed using larger sample populations.

KEYWORDS:

Saliva; amylase; cystatins; gel electrophoresis; mass spectrometry; stress

PMID:
28055279
PMCID:
PMC5551674
[Available on 2018-05-01]
DOI:
10.1080/1354750X.2017.1279215
[Indexed for MEDLINE]
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