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Biomed Res Int. 2016;2016:1917394. doi: 10.1155/2016/1917394. Epub 2016 Dec 7.

Proteomic Profiling of Neuroblastoma Cells Adhesion on Hyaluronic Acid-Based Surface for Neural Tissue Engineering.

Author information

1
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
2
Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan.
3
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
4
Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
5
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan; Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
6
Office of Safety, Health and Environment, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
7
Mass Spectrometry Laboratory, Chemical, Molecular and Materials Analysis Center, Department of Chemistry, National University of Singapore, Singapore 119077.
8
Department of Biomedical Engineering, Chung Yuan Christian University, Chungli 300, Taiwan.
9
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
10
Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
11
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.

Abstract

The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid (HA) biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media (PC12 CM) in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS/MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.

PMID:
28053978
PMCID:
PMC5174748
DOI:
10.1155/2016/1917394
[Indexed for MEDLINE]
Free PMC Article

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