Format

Send to

Choose Destination
ACS Sens. 2016 Nov 23;1(11):1366-1373. doi: 10.1021/acssensors.6b00533. Epub 2016 Nov 14.

Oxygen Sensing Difluoroboron β-Diketonate Polylactide Materials with Tunable Dynamic Ranges for Wound Imaging.

Author information

1
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904.
2
Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908.
3
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904; Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908.

Abstract

Difluoroboron β-diketonate poly(lactic acid) materials exhibit both fluorescence (F) and oxygen sensitive room-temperature phosphorescence (RTP). Introduction of halide heavy atoms (Br and I) is an effective strategy to control the oxygen sensitivity in these materials. A series of naphthyl-phenyl (nbm) dye derivatives with hydrogen, bromide and iodide substituents were prepared for comparison. As nanoparticles, the hydrogen derivative was hypersensitive to oxygen (0-0.3%), while the bromide analogue was suited for hypoxia detection (0-3% O2). The iodo derivative, BF2nbm(I)PLA, showed excellent F to RTP peak separation and an 0-100% oxygen sensitivity range unprecedented for metal-free RTP emitting materials. Due to the dual emission and unconventionally long RTP lifetimes of these O2 sensing materials, a portable, cost-effective camera was used to quantify oxygen levels via lifetime and red/green/blue (RGB) ratiometry. The hypersensitive H dye was well matched to lifetime detection, simultaneous lifetime and ratiometric imaging was possible for the bromide analogue, whereas the iodide material, with intense RTP emission and a shorter lifetime, was suited for RGB ratiometry. To demonstrate the prospects of this camera/material design combination for bioimaging, iodide boron dye-PLA nanoparticles were applied to a murine wound model to detect oxygen levels. Surprisingly, wound oxygen imaging was achieved without covering (i.e. without isolating from ambient conditions, air). Additionally, would healing was monitored via wound size reduction and associated oxygen recovery, from hypoxic to normoxic. These single-component materials provide a simple tunable platform for biological oxygen sensing that can be deployed to spatially resolve oxygen in a variety of environments.

KEYWORDS:

Difluoroboron β–diketonate complexes; RGB camera imaging; fluorescence; poly(lactic acid); ratiometric oxygen sensing; room-temperature phosphorescence; wound healing

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center