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J Lab Physicians. 2017 Jan-Mar;9(1):53-56. doi: 10.4103/0974-2727.187929.

A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes.

Author information

1
Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
2
Department of Dermatology and STD, Maulana Azad Medical College, New Delhi, India.

Abstract

OBJECTIVE:

To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes.

MATERIALS AND METHODS:

A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared.

RESULTS:

PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases.

CONCLUSION:

PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

KEYWORDS:

Genital herpes; herpes simplex virus polymerase chain reaction; simultaneous detection and typing of herpes simplex virus

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