Format

Send to

Choose Destination
Stem Cell Reports. 2017 Jan 10;8(1):21-29. doi: 10.1016/j.stemcr.2016.12.001. Epub 2016 Dec 29.

SNEVhPrp19/hPso4 Regulates Adipogenesis of Human Adipose Stromal Cells.

Author information

1
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Department of Bioinformatics and Biotechnology of Faculty of Basic and Applied Sciences, International Islamic University, 44000 Islamabad, Pakistan.
2
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Christian Doppler Laboratory on Biotechnology of Skin Aging, 1190 Vienna, Austria.
3
Department of Phoniatrics, ENT University Hospital, Medical University of Graz, 8036 Graz, Austria.
4
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
5
Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; Red Cross Blood Transfusion Service of Upper Austria, 4017 Linz, Austria.
6
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria.
7
Research Institute for Biomedical Aging Research, Center for Molecular Biosciences (CMBI), University of Innsbruck, 6020 Innsbruck, Austria.
8
Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, 1200 Vienna, Austria.
9
Institute for Diabetes and Cancer (IDC), Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany; Joint Heidelberg-IDC Translational Diabetes Program, Heidelberg University Hospital, 69120 Heidelberg, Germany; Molecular Metabolic Control, Medical Faculty, Technical University Munich, 80333 Munich, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany.
10
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: markus.schosserer@boku.ac.at.
11
Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Christian Doppler Laboratory on Biotechnology of Skin Aging, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: johannes.grillari@boku.ac.at.

Abstract

Aging is accompanied by loss of subcutaneous adipose tissue. This may be due to reduced differentiation capacity or deficiency in DNA damage repair (DDR) factors. Here we investigated the role of SNEVhPrp19/hPso4, which was implicated in DDR and senescence evasion, in adipogenic differentiation of human adipose stromal cells (hASCs). We showed that SNEV is induced during adipogenesis and localized both in the nucleus and in the cytoplasm. Knockdown of SNEV perturbed adipogenic differentiation and led to accumulation of DNA damage in hASCs upon oxidative stress. In addition, we demonstrated that SNEV is required for fat deposition in Caenorhabditis elegans. Consequently, we tested other DDR factors and found that WRN is also required for adipogenesis in both models. These results demonstrate that SNEV regulates adipogenesis in hASCs and indicate that DDR capacity in general might be a pre-requisite for this process.

KEYWORDS:

C. elegans; DNA damage repair; Prp19; Pso4; SNEV; WRN; adipogenesis; human adipose stromal cells

PMID:
28041875
PMCID:
PMC5233435
DOI:
10.1016/j.stemcr.2016.12.001
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center