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Genome Biol Evol. 2016 Dec 31;8(11):3574-3599. doi: 10.1093/gbe/evw259.

Comparative "Omics" of the Fusarium fujikuroi Species Complex Highlights Differences in Genetic Potential and Metabolite Synthesis.

Author information

1
Institut für Biologie und Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Münster, Germany.
2
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Neuherberg, Germany.
3
United States Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, Illinois.
4
Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel.
5
Department of Energy Joint Genome Institute, University of California, Walnut Creek, Berkeley, California.
6
Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
7
Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel.
8
Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
9
University of Amsterdam, Swammerdam Institute for Life Sciences, Plant Pathology, Amsterdam, The Netherlands.
10
Rheinische Friedrich-Wilhelms-Universität Bonn, Kekulé-Institut für Organische Chemie und Biochemie, Germany.
11
Department of Microbiology Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
12
Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Maximus-von-Imhof-Forum 3, Freising, Germany tudzynsb@uni-muenster.de u.gueldener@tum.de.
13
Institut für Biologie und Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Münster, Germany tudzynsb@uni-muenster.de u.gueldener@tum.de.

Abstract

Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum of often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although species within the FFC are difficult to distinguish by morphology, and their genes often share 90% sequence similarity, they can differ in host plant specificity and life style. FFC species can also produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin, and the phytohormones gibberellins, auxins, and cytokinins. The spectrum of SMs produced can differ among closely related species, suggesting that SMs might be determinants of host specificity. To date, genomes of only a limited number of FFC species have been sequenced. Here, we provide draft genome sequences of three more members of the FFC: a single isolate of F. mangiferae, the cause of mango malformation, and two isolates of F. proliferatum, one a pathogen of maize and the other an orchid endophyte. We compared these genomes to publicly available genome sequences of three other FFC species. The comparisons revealed species-specific and isolate-specific differences in the composition and expression (in vitro and in planta) of genes involved in SM production including those for phytohormome biosynthesis. Such differences have the potential to impact host specificity and, as in the case of F. proliferatum, the pathogenic versus endophytic life style.

KEYWORDS:

Fusarium fujikuroi species complex; evolution; genome sequencing; in planta expression; metabolomics; secondary metabolism

PMID:
28040774
PMCID:
PMC5203792
DOI:
10.1093/gbe/evw259
[Indexed for MEDLINE]
Free PMC Article

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