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Nutrients. 2016 Dec 29;9(1). pii: E10. doi: 10.3390/nu9010010.

Methionine and Choline Supply during the Periparturient Period Alter Plasma Amino Acid and One-Carbon Metabolism Profiles to Various Extents: Potential Role in Hepatic Metabolism and Antioxidant Status.

Author information

1
Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA. zzhou32@illinois.edu.
2
Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA. vailati2@illinois.edu.
3
Adisseo North America, Alpharetta, GA 30022, USA. daniel.luchini@adisseo.com.
4
Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA. jloor@illinois.edu.

Abstract

The objective of this study was to profile plasma amino acids (AA) and derivatives of their metabolism during the periparturient period in response to supplemental rumen-protected methionine (MET) or rumen-protected choline (CHOL). Forty cows were fed from -21 through 30 days around parturition in a 2 × 2 factorial design a diet containing MET or CHOL. MET supply led to greater circulating methionine and proportion of methionine in the essential AA pool, total AA, and total sulfur-containing compounds. Lysine in total AA also was greater in these cows, indicating a better overall AA profile. Sulfur-containing compounds (cystathionine, cystine, homocystine, and taurine) were greater in MET-fed cows, indicating an enriched sulfur-containing compound pool due to enhanced transsulfuration activity. Circulating essential AA and total AA concentrations were greater in cows supplied MET due to greater lysine, arginine, tryptophan, threonine, proline, asparagine, alanine, and citrulline. In contrast, CHOL supply had no effect on essential AA or total AA, and only tryptophan and cystine were greater. Plasma 3-methylhistidine concentration was lower in response to CHOL supply, suggesting less tissue protein mobilization in these cows. Overall, the data revealed that enhanced periparturient supply of MET has positive effects on plasma AA profiles and overall antioxidant status.

KEYWORDS:

amino acids; lactation; methyl donors; periparturient dairy cow

PMID:
28036059
PMCID:
PMC5295054
DOI:
10.3390/nu9010010
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors have read the journal’s policy and one of the authors of this manuscript has the following competing interests: Daniel N. Luchini is an employee of Adisseo USA Inc. (Alpharetta, GA, USA). Adisseo USA Inc. had a role in the study design and provided financial support to cover costs of animal use, data collection, and sample analyses.

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