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Mol Cells. 2016 Dec;39(12):841-846. doi: 10.14348/molcells.2016.0277. Epub 2016 Dec 29.

Imaging Single-mRNA Localization and Translation in Live Neurons.

Author information

1
Department of Physics and Astronomy, Seoul National University, Seoul 08826, Korea.
2
Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea.
3
The Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea.

Abstract

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

KEYWORDS:

MS2-GFP; RNA localization; live-cell imaging; local translation; single-molecule imaging

PMID:
28030897
PMCID:
PMC5223100
DOI:
10.14348/molcells.2016.0277
[Indexed for MEDLINE]
Free PMC Article

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