Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):E219-E227. doi: 10.1073/pnas.1611684114. Epub 2016 Dec 27.

Mosaic expression of claudins in thick ascending limbs of Henle results in spatial separation of paracellular Na+ and Mg2+ transport.

Author information

1
Institute of Physiology, Christian-Albrechts-University of Kiel, 24098 Kiel, Germany; s.milatz@physiologie.uni-kiel.de.
2
Institute of Physiology, Christian-Albrechts-University of Kiel, 24098 Kiel, Germany.
3
Department of Nephrology and Hypertension, Hannover Medical School, 30625 Hannover, Germany.
4
Department of Anatomy, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
5
Renal Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
6
Institute of Clinical Physiology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
7
Department of Pediatric Nephrology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.

Abstract

The thick ascending limb (TAL) of Henle's loop drives paracellular Na+, Ca2+, and Mg2+ reabsorption via the tight junction (TJ). The TJ is composed of claudins that consist of four transmembrane segments, two extracellular segments (ECS1 and -2), and one intracellular loop. Claudins interact within the same (cis) and opposing (trans) plasma membranes. The claudins Cldn10b, -16, and -19 facilitate cation reabsorption in the TAL, and their absence leads to a severe disturbance of renal ion homeostasis. We combined electrophysiological measurements on microperfused mouse TAL segments with subsequent analysis of claudin expression by immunostaining and confocal microscopy. Claudin interaction properties were examined using heterologous expression in the TJ-free cell line HEK 293, live-cell imaging, and Förster/FRET. To reveal determinants of interaction properties, a set of TAL claudin protein chimeras was created and analyzed. Our main findings are that (i) TAL TJs show a mosaic expression pattern of either cldn10b or cldn3/cldn16/cldn19 in a complex; (ii) TJs dominated by cldn10b prefer Na+ over Mg2+, whereas TJs dominated by cldn16 favor Mg2+ over Na+; (iii) cldn10b does not interact with other TAL claudins, whereas cldn3 and cldn16 can interact with cldn19 to form joint strands; and (iv) further claudin segments in addition to ECS2 are crucial for trans interaction. We suggest the existence of at least two spatially distinct types of paracellular channels in TAL: a cldn10b-based channel for monovalent cations such as Na+ and a spatially distinct site for reabsorption of divalent cations such as Ca2+ and Mg2.

KEYWORDS:

FRET; claudin interaction; microperfusion; paracellular ion transport; tight junction

PMID:
28028216
PMCID:
PMC5240732
DOI:
10.1073/pnas.1611684114
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center