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Bioelectromagnetics. 2017 Apr;38(3):175-185. doi: 10.1002/bem.22032. Epub 2016 Dec 27.

RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.

Su L1,2, Wei X1, Xu Z1,2, Chen G1,2.

Author information

1
Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.
2
Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.

Abstract

Despite many years of studies, the debate on genotoxic effects of radiofrequency electromagnetic fields (RF-EMF) continues. To systematically evaluate genotoxicity of RF-EMF, this study examined effects of RF-EMF on DNA damage and cellular behavior in different neurogenic cells. Neurogenic A172, U251, and SH-SY5Y cells were intermittently (5 min on/10 min off) exposed to 1800 MHz RF-EMF at an average specific absorption rate (SAR) of 4.0 W/kg for 1, 6, or 24 h. DNA damage was evaluated by quantification of γH2AX foci, an early marker of DNA double-strand breaks. Cell cycle progression, cell proliferation, and cell viability were examined by flow cytometry, hemocytometer, and cell counting kit-8 assay, respectively. Results showed that exposure to RF-EMF at an SAR of 4.0 W/kg neither significantly induced γH2AX foci formation in A172, U251, or SH-SY5Y cells, nor resulted in abnormal cell cycle progression, cell proliferation, or cell viability. Furthermore, prolonged incubation of these cells for up to 48 h after exposure did not significantly affect cellular behavior. Our data suggest that 1800 MHz RF-EMF exposure at 4.0 W/kg is unlikely to elicit DNA damage or abnormal cellular behaviors in neurogenic cells. Bioelectromagnetics. 38:175-185, 2017.

KEYWORDS:

DNA damage; cell cycle; cell proliferation; cell viability; mobile phone

PMID:
28026047
DOI:
10.1002/bem.22032
[Indexed for MEDLINE]

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