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Biochem Biophys Res Commun. 2017 Jan 29;483(1):288-293. doi: 10.1016/j.bbrc.2016.12.150. Epub 2016 Dec 23.

MafB is required for development of the hindbrain choroid plexus.

Author information

1
Department of Anatomy and Neuroscience, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan. Electronic address: rkoshida-myz@umin.ac.jp.
2
Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
3
Department of Anatomy and Neuroscience, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.

Abstract

The choroid plexus (ChP) is a non-neural epithelial tissue that produces cerebrospinal fluid (CSF). The ChP differentiates from the roof plate, a dorsal midline structure of the neural tube. However, molecular mechanisms underlying ChP development are poorly understood compared to neural development. MafB is a bZip transcription factor that is known to be expressed in the roof plate. Here we investigated the role of MafB in embryonic development of the hindbrain ChP (hChP) using Mafb-deficient mice. Immunohistochemical analyses revealed that MafB is expressed in the roof plate and early hChP epithelial cells but its expression disappears at a later embryonic stage. We also found that the Mafb-deficient hChP exhibits delayed differentiation and results in hypoplasia compared to the wild-type hChP. Furthermore, the Mafb-deficient hChP exhibits increased apoptotic cell death and decreased proliferating cells at E12.5, an early stage of hChP development. Collectively, our findings reveal that MafB play an important role in promoting hChP development during embryogenesis.

KEYWORDS:

Hindbrain choroid plexus; Lmx1a; MafB; Roof plate

PMID:
28025141
DOI:
10.1016/j.bbrc.2016.12.150
[Indexed for MEDLINE]

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