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Anal Chem. 1989 Sep 15;61(18):2009-15.

Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy.

Erratum in

  • Anal Chem 1995 Feb 15;67(4):775.


A spectral analysis of whole EDTA blood was undertaken by using attenuated total reflection and Fourier-transform infrared spectroscopy. The concentration of blood glucose was measured by an enzymatic method using glucose dehydrogenase and ranged between 40 and 290 mg/dL with an average concentration of 90.4 mg/dL. Multivariate calibration with the partial least-squares (PLS) algorithm was performed on spectral data between 1500 and 750 cm-1 showing a varying background from different unidentified interfering compounds. Cross validation was carried out for optimizing the PLS model. PRESS was 19.8 mg/dL, which was calculated on the basis of 127 standards, whereas the estimated standard deviation for the calibration fit was computed to be 11.9 mg/dL. Infrared spectroscopy can be used for monitoring glucose levels within the normal physiological range in a complex matrix like whole blood as an alternative to electrochemical sensors.

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