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Biomed Res Int. 2016;2016:9014364. doi: 10.1155/2016/9014364. Epub 2016 Nov 28.

Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway.

Author information

1
Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; School of Life Sciences, Jilin University, Jilin 130012, China.
2
Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; Southern Research Institute, Jilin University, Zhuhai 519000, China.
3
School of Life Sciences, Jilin University, Jilin 130012, China.

Abstract

Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities of T. matsutake aqueous extract (TM) in HepG2 and SMMC-7721 cells. In in vitro experiments, TM strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ROS, and caused caspases cascade and mitochondrial membrane potential dissipation in hepatocellular carcinoma cells. In in vivo experiments, 14-day TM treatment strongly suppressed tumor growth in HepG2 and SMMC-7721-xenografted nude mice without influence on their body weights and liver function. Furthermore, TM increased the levels of cleaved poly-ADP-ribose polymerase (PARP), Bad, and Bax and reduced the expressions of B-cell lymphoma 2 (Bcl-2) in treated cells and tumor tissues. All aforementioned results suggest that caspase-dependent mitochondrial apoptotic pathways are involved in TM-mediated antihepatocellular carcinoma.

PMID:
28018916
PMCID:
PMC5149606
DOI:
10.1155/2016/9014364
[Indexed for MEDLINE]
Free PMC Article

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