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Bioinformatics. 2017 Mar 1;33(5):751-753. doi: 10.1093/bioinformatics/btw741.

SeqLib: a C ++ API for rapid BAM manipulation, sequence alignment and sequence assembly.

Wala J1,2,3, Beroukhim R1,2,3.

Author information

1
The Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
2
Bioinformatics and Integrative Genomics, Harvard University, Cambridge, MA 02138, USA.
3
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Abstract

We present SeqLib, a C ++ API and command line tool that provides a rapid and user-friendly interface to BAM/SAM/CRAM files, global sequence alignment operations and sequence assembly. Four C libraries perform core operations in SeqLib: HTSlib for BAM access, BWA-MEM and BLAT for sequence alignment and Fermi for error correction and sequence assembly. Benchmarking indicates that SeqLib has lower CPU and memory requirements than leading C ++ sequence analysis APIs. We demonstrate an example of how minimal SeqLib code can extract, error-correct and assemble reads from a CRAM file and then align with BWA-MEM. SeqLib also provides additional capabilities, including chromosome-aware interval queries and read plotting. Command line tools are available for performing integrated error correction, micro-assemblies and alignment.

Availability and Implementation:

SeqLib is available on Linux and OSX for the C ++98 standard and later at github.com/walaj/SeqLib. SeqLib is released under the Apache2 license. Additional capabilities for BLAT alignment are available under the BLAT license.

Contact:

jwala@broadinstitue.org ; rameen@broadinstitute.org.

PMID:
28011768
PMCID:
PMC5859992
DOI:
10.1093/bioinformatics/btw741
[Indexed for MEDLINE]
Free PMC Article

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