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J Biol Chem. 2017 Feb 10;292(6):2217-2225. doi: 10.1074/jbc.M116.764209. Epub 2016 Dec 23.

Deciphering Key Residues Involved in the Virulence-promoting Interactions between Streptococcus pneumoniae and Human Plasminogen.

Author information

1
From the Institut de Biologie Structurale (IBS), Université Grenoble Alpes, Commissariat à l'Energie Atomique (CEA), CNRS, 38044 Grenoble, France.
2
From the Institut de Biologie Structurale (IBS), Université Grenoble Alpes, Commissariat à l'Energie Atomique (CEA), CNRS, 38044 Grenoble, France christine.gaboriaud@ibs.fr.
3
From the Institut de Biologie Structurale (IBS), Université Grenoble Alpes, Commissariat à l'Energie Atomique (CEA), CNRS, 38044 Grenoble, France anne-marie.di-guilmi@ibs.fr.

Abstract

Bacterial pathogens recruit circulating proteins to their own surfaces, co-opting the host protein functions as a mechanism of virulence. Particular attention has focused on the binding of plasminogen (Plg) to bacterial surfaces, as it has been shown that this interaction contributes to bacterial adhesion to host cells, invasion of host tissues, and evasion of the immune system. Several bacterial proteins are known to serve as receptors for Plg including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cytoplasmic enzyme that appears on the cell surface in this moonlighting role. Although Plg typically binds to these receptors via several lysine-binding domains, the specific interactions that occur have not been documented in all cases. However, identification of the relevant residues could help define strategies for mitigating the virulence of important human pathogens, such as Streptococcus pneumoniae (Sp). To shed light on this question, we have described a combination of peptide-spot array screening, competition and SPR assays, high-resolution crystallography, and mutational analyses to characterize the interaction between SpGAPDH and Plg. We identified three SpGAPDH lysine residues that were instrumental in defining the kinetic and thermodynamic parameters of the interaction. Altogether, the integration of the data presented in this work allows us to propose a structural model for the molecular interaction of the SpGAPDH-Plg complex.

KEYWORDS:

Streptococcus; bacterial pathogenesis; binding site mapping; cell surface receptor; crystal structure; gram-positive bacteria; host-pathogen interaction; human plasminogen; peptide array; plasminogen; pneumococcal GAPDH; protein-protein interaction

PMID:
28011643
PMCID:
PMC5313095
DOI:
10.1074/jbc.M116.764209
[Indexed for MEDLINE]
Free PMC Article

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