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EMBO J. 2017 Feb 1;36(3):301-318. doi: 10.15252/embj.201695513. Epub 2016 Dec 23.

Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter.

Author information

1
Microbial Sciences Institute, Yale University, West Haven, CT, USA.
2
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.
3
Howard Hughes Medical Institute, Yale University, New Haven, CT, USA.
4
Microbial Sciences Institute, Yale University, West Haven, CT, USA christine.jacobs-wagner@yale.edu.
5
Department of Microbial Pathogenesis, Yale Medical School, Yale University, New Haven, CT, USA.

Abstract

In bacteria, chromosome dynamics and gene expression are modulated by nucleoid-associated proteins (NAPs), but little is known about how NAP activity is coupled to cell cycle progression. Using genomic techniques, quantitative cell imaging, and mathematical modeling, our study in Caulobacter crescentus identifies a novel NAP (GapR) whose activity over the cell cycle is shaped by DNA replication. GapR activity is critical for cellular function, as loss of GapR causes severe, pleiotropic defects in growth, cell division, DNA replication, and chromosome segregation. GapR also affects global gene expression with a chromosomal bias from origin to terminus, which is associated with a similar general bias in GapR binding activity along the chromosome. Strikingly, this asymmetric localization cannot be explained by the distribution of GapR binding sites on the chromosome. Instead, we present a mechanistic model in which the spatiotemporal dynamics of GapR are primarily driven by the progression of the replication forks. This model represents a simple mechanism of cell cycle regulation, in which DNA-binding activity is intimately linked to the action of DNA replication.

KEYWORDS:

Caulobacter ; DNA replication; cell cycle; chromosome organization; nucleoid‐associated protein

PMID:
28011580
PMCID:
PMC5286365
DOI:
10.15252/embj.201695513
[Indexed for MEDLINE]
Free PMC Article

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